Team Logo

ACT

:: Analysis of Common Targets for circular RNAs ::

NCHU_Logo

The Laboratory of
X-omics

ACT files Back to home
user_sequence.txt The original inputted sequence.
user_sequence_backspliceJCT.txt The sequence of backsplice junction generated from the first and last 30 nucleotides of inputted sequences.
user_miranda_output.txt
user_miranda_output_bJCT.txt
Raw results from miRanda.
user_pmiranda.txt from original seq Parsed miRanda results in table form.
user_pmiranda_bJCT_raw.txt from backsplice junction
user_pmiranda_bJCT.txt non-redudant results
user_ACT_table.txt

Complete output of ACT (Columns separated by ‘tab’):
Each row represents a circular RNA. There are 4 columns. Column 1: sequence ID. Column 2: List of gene symbols of circRNA targets, ranked by targeting time and separated by semicolon “;”. Column 3: List of common targeting time of circRNA targets, ranked by targeting time and separated by semicolon “;”. These numbers are paired with the gene symbols listed in the column 2. Column 4: List of miRNAs with binding sites found in the given circRNA sequence.

user_ACT_report.txt

Summary of ACT for each circRNA, shown as a block. Column description:
circRNA: names of inputted sequence
length (nt): length of the inputted sequence
#miRNAs: number of unique miRNAs
#miRNA-binding sites: number of miRNA binding sites found on the circRNA
miR-sites/100nt: number of miRNA binding sites found on the circRNA per 100 nt
#targets: total number of target genes
Common Targets (targeting #): gene symbol (number of targeting time)
List of Common Targets [Gene Symbol Only]: gene symbols of target genes

user_ACT_list.gmt Top 100 candidate genes exported as gmt format.
user_ACT.log Log file for monitoring the progress of analysis.
[ACT]: ACT is done.
Please use lab.x.ometics@gmail.com to report problems or leave comments

2018 Laboratory of X-omics

:: This server is made possible by funding from National Chung Hsing University, Hsing-Chun Project and MOST of Taiwan ::