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miranda v3.3a    microRNA Target Scanning Algorithm
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(c) 2003 Memorial Sloan-Kettering Cancer Center, New York

Authors: Anton Enright, Bino John, Chris Sander and Debora Marks
(mirnatargets (at) cbio.mskcc.org - reaches all authors)

Software written by: Anton Enright
Distributed for anyone to use under the GNU Public License (GPL),
See the files 'COPYING' and 'LICENSE' for details

If you use this software please cite:
Enright AJ, John B, Gaul U, Tuschl T, Sander C and Marks DS;
(2003) Genome Biology; 5(1):R1.

   miranda comes with ABSOLUTELY NO WARRANTY;
   This is free software, and you are welcome to redistribute it
   under certain conditions; type `miranda --license' for details.

Current Settings:
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Query Filename: 	miRBase_hsa_mature_2018-03-29.fa
Reference Filename:	/var/www/html/ACT_Server/out/125ABC84A31C676/user_sequence_backspliceJCT.txt
Gap Open Penalty:	-9.000000
Gap Extend Penalty:	-4.000000
Score Threshold:	140.000000
Energy Threshold:	1.000000 kcal/mol
Scaling Parameter:	4.000000
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   Forward:	Score: 166.000000  Q:2 to 20  R:18 to 40 Align Len (19) (73.68%) (89.47%)

   Query:    3' acuCCGUCGUGU-UAUACCGGAc 5'
                   ||:| :||| :|||||||| 
   Ref:      5' ctcGGTACTACAGGTATGGCCTc 3'

   Energy:  -22.040001 kCal/Mol

Scores for this hit:
>hsa-miR-15a-3p	HIPK3_2_2	166.00	-22.04	2 20	18 40	19	73.68%	89.47%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-15a-3p	HIPK3_2_2	166.00	-22.04	166.00	-22.04	16	22	60	 18
Complete


   Forward:	Score: 154.000000  Q:2 to 21  R:35 to 59 Align Len (21) (66.67%) (80.95%)

   Query:    3' ggaGGGUG-ACGGGA-GAGAUGGGc 5'
                   |:|||  |:|:|  ||||||| 
   Ref:      5' ggcCTCACAAGTCTTGGTCTACCCa 3'

   Energy:  -24.959999 kCal/Mol

Scores for this hit:
>hsa-miR-197-5p	HIPK3_2_2	154.00	-24.96	2 21	35 59	21	66.67%	80.95%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-197-5p	HIPK3_2_2	154.00	-24.96	154.00	-24.96	92	23	60	 35
Complete


   Forward:	Score: 148.000000  Q:2 to 13  R:1 to 12 Align Len (11) (81.82%) (90.91%)

   Query:    3' uggauuauauAGUUUGUAUAGu 5'
                          |: |||||||| 
   Ref:      5' ----------TTCAACATATCt 3'

   Energy:  -8.220000 kCal/Mol

Scores for this hit:
>hsa-miR-190a-5p	HIPK3_2_2	148.00	-8.22	2 13	1 12	11	81.82%	90.91%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-190a-5p	HIPK3_2_2	148.00	-8.22	148.00	-8.22	252	22	60	 1
Complete


   Forward:	Score: 150.000000  Q:2 to 19  R:38 to 58 Align Len (17) (64.71%) (76.47%)

   Query:    3' ggaUGCAAGGUAUCAGAUGGu 5'
                   ||   :| |:||||||| 
   Ref:      5' ctcACAAGTCTTGGTCTACCc 3'

   Energy:  -15.860000 kCal/Mol

Scores for this hit:
>hsa-miR-379-5p	HIPK3_2_2	150.00	-15.86	2 19	38 58	17	64.71%	76.47%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-379-5p	HIPK3_2_2	150.00	-15.86	150.00	-15.86	338	21	60	 38
Complete


   Forward:	Score: 153.000000  Q:2 to 18  R:10 to 31 Align Len (16) (68.75%) (81.25%)

   Query:    3' gaccuUCACGGGUAUGAUGUCa 5'
                     | | :| :|||||||| 
   Ref:      5' tctacAATCTCGGTACTACAGg 3'

   Energy:  -15.830000 kCal/Mol

Scores for this hit:
>hsa-miR-20b-3p	HIPK3_2_2	153.00	-15.83	2 18	10 31	16	68.75%	81.25%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-20b-3p	HIPK3_2_2	153.00	-15.83	153.00	-15.83	395	22	60	 10
Complete


   Forward:	Score: 153.000000  Q:2 to 19  R:1 to 19 Align Len (18) (72.22%) (77.78%)

   Query:    3' agaugAGGUU-UUCCGAUGUUAGu 5'
                     |:||| |   |||||||| 
   Ref:      5' -----TTCAACATATCTACAATCt 3'

   Energy:  -11.630000 kCal/Mol

Scores for this hit:
>hsa-miR-508-3p	HIPK3_2_2	153.00	-11.63	2 19	1 19	18	72.22%	77.78%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-508-3p	HIPK3_2_2	153.00	-11.63	153.00	-11.63	543	23	60	 1
Complete


   Forward:	Score: 140.000000  Q:2 to 9  R:1 to 8 Align Len (7) (100.00%) (100.00%)

   Query:    3' gucaucucuaacaAAGUUGUg 5'
                             ||||||| 
   Ref:      5' -------------TTCAACAt 3'

   Energy:  -6.980000 kCal/Mol

Scores for this hit:
>hsa-miR-653-5p	HIPK3_2_2	140.00	-6.98	2 9	1 8	7	100.00%	100.00%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-653-5p	HIPK3_2_2	140.00	-6.98	140.00	-6.98	709	21	60	 1
Complete


   Forward:	Score: 148.000000  Q:2 to 13  R:1 to 12 Align Len (11) (81.82%) (90.91%)

   Query:    3' guuggguuauAGUUUGUAUAGu 5'
                          |: |||||||| 
   Ref:      5' ----------TTCAACATATCt 3'

   Energy:  -8.220000 kCal/Mol

Scores for this hit:
>hsa-miR-190b-5p	HIPK3_2_2	148.00	-8.22	2 13	1 12	11	81.82%	90.91%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-190b-5p	HIPK3_2_2	148.00	-8.22	148.00	-8.22	807	22	60	 1
Complete


   Forward:	Score: 145.000000  Q:2 to 19  R:32 to 60 Align Len (22) (59.09%) (72.73%)

   Query:    3' gcgagaGGGGGUUUGG-----GAUGGGUg 5'
                      |:| |||::|     ||||||| 
   Ref:      5' tatggcCTCACAAGTCTTGGTCTACCCAc 3'

   Energy:  -26.340000 kCal/Mol

Scores for this hit:
>hsa-miR-1229-5p	HIPK3_2_2	145.00	-26.34	2 19	32 60	22	59.09%	72.73%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-1229-5p	HIPK3_2_2	145.00	-26.34	145.00	-26.34	868	24	60	 32
Complete


   Forward:	Score: 158.000000  Q:2 to 23  R:36 to 59 Align Len (21) (66.67%) (71.43%)

   Query:    3' agGAGACUCGAGGAAGAGAUGGGu 5'
                  |||  |  ||:|  ||||||| 
   Ref:      5' gcCTCACAAGTCTTGGTCTACCCa 3'

   Energy:  -26.990000 kCal/Mol

Scores for this hit:
>hsa-miR-3132	HIPK3_2_2	158.00	-26.99	2 23	36 59	21	66.67%	71.43%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-3132	HIPK3_2_2	158.00	-26.99	158.00	-26.99	1068	24	60	 36
Complete


   Forward:	Score: 149.000000  Q:2 to 15  R:25 to 43 Align Len (15) (80.00%) (80.00%)

   Query:    3' gugGUCGAU--CGGAGUGg 5'
                   ||| ||  ||||||| 
   Ref:      5' ctaCAGGTATGGCCTCACa 3'

   Energy:  -20.299999 kCal/Mol

Scores for this hit:
>hsa-miR-4316	HIPK3_2_2	149.00	-20.30	2 15	25 43	15	80.00%	80.00%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-4316	HIPK3_2_2	149.00	-20.30	149.00	-20.30	1207	17	60	 25
Complete


   Forward:	Score: 142.000000  Q:2 to 21  R:1 to 17 Align Len (19) (68.42%) (73.68%)

   Query:    3' ccGGUUAGAAUCCAAGAUGUUa 5'
                  :||| | ||   ||||||| 
   Ref:      5' -tTCAA-CATA---TCTACAAt 3'

   Energy:  -9.710000 kCal/Mol

Scores for this hit:
>hsa-miR-3674	HIPK3_2_2	142.00	-9.71	2 21	1 17	19	68.42%	73.68%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-3674	HIPK3_2_2	142.00	-9.71	142.00	-9.71	1323	22	60	 1
Complete


   Forward:	Score: 142.000000  Q:2 to 11  R:1 to 15 Align Len (9) (88.89%) (88.89%)

   Query:    3' caccgaccacgaGCAUAGAUGu 5'
                            | ||||||| 
   Ref:      5' -------ttcaaCATATCTACa 3'

   Energy:  -9.720000 kCal/Mol

Scores for this hit:
>hsa-miR-3935	HIPK3_2_2	142.00	-9.72	2 11	1 15	9	88.89%	88.89%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-3935	HIPK3_2_2	142.00	-9.72	142.00	-9.72	1391	22	60	 1
Complete


   Forward:	Score: 140.000000  Q:2 to 9  R:4 to 24 Align Len (7) (100.00%) (100.00%)

   Query:    3' ugucggcuccgggAGAGCCAg 5'
                             ||||||| 
   Ref:      5' aacatatctacaaTCTCGGTa 3'

   Energy:  -14.900000 kCal/Mol

Scores for this hit:
>hsa-miR-4523	HIPK3_2_2	140.00	-14.90	2 9	4 24	7	100.00%	100.00%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-4523	HIPK3_2_2	140.00	-14.90	140.00	-14.90	1553	21	60	 4
Complete


   Forward:	Score: 174.000000  Q:2 to 19  R:37 to 58 Align Len (17) (76.47%) (100.00%)

   Query:    3' uuguUGUUUAGGGUCAGAUGGa 5'
                    ||||:||:::||||||| 
   Ref:      5' cctcACAAGTCTTGGTCTACCc 3'

   Energy:  -25.459999 kCal/Mol

Scores for this hit:
>hsa-miR-3529-5p	HIPK3_2_2	174.00	-25.46	2 19	37 58	17	76.47%	100.00%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-3529-5p	HIPK3_2_2	174.00	-25.46	174.00	-25.46	1727	22	60	 37
Complete


   Forward:	Score: 158.000000  Q:2 to 17  R:2 to 21 Align Len (15) (86.67%) (86.67%)

   Query:    3' uggacUAAAGAUGGUUAGAGa 5'
                     || |||| ||||||| 
   Ref:      5' tcaacATATCTA-CAATCTCg 3'

   Energy:  -12.500000 kCal/Mol

Scores for this hit:
>hsa-miR-5186	HIPK3_2_2	158.00	-12.50	2 17	2 21	15	86.67%	86.67%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-5186	HIPK3_2_2	158.00	-12.50	158.00	-12.50	1927	21	60	 2
Complete


   Forward:	Score: 151.000000  Q:2 to 20  R:28 to 50 Align Len (18) (61.11%) (77.78%)

   Query:    3' ggggACAUUAGGUUCUUCAGAAg 5'
                    | |:: |: | ||||||| 
   Ref:      5' caggTATGGCCTCACAAGTCTTg 3'

   Energy:  -12.630000 kCal/Mol

Scores for this hit:
>hsa-miR-8087	HIPK3_2_2	151.00	-12.63	2 20	28 50	18	61.11%	77.78%

Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions
>>hsa-miR-8087	HIPK3_2_2	151.00	-12.63	151.00	-12.63	2573	23	60	 28
Complete

Scan Complete